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tsaE [2020-10-13 17:26:40]
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tsaE [2020-10-13 17:26:40]

protein kinase, required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA
Locus
BSU_05910
Isoelectric point
4.60
Molecular weight
17.76 kDa
Protein length
158 aa Sequence Blast
Gene length
477 bp Sequence Blast
Function
control of tRNA modification
Product
protein kinase involved in tRNA modification
Essential
no
Synonyms
ydiB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation
  • Information processing → Protein synthesis, modification and degradation → Protein modification → Protein kinases
  • Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

    • Gene

    Coordinates
    641,654 → 642,130

    Phenotypes of a mutant

  • reduced growth rate PubMed
  • increased sensitivity to paraquat PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP (TsaB-TsaD-TsaE) PubMed
  • autophosphorylation on Ser-60, Thr-55, Thr-62, and Thr-64 PubMed
  • phosphorylation of TsaD PubMed

    • Protein family

  • TsaE family (single member, according to UniProt)

    • Kinetic information

  • K(M) for ATP: 60 myM, V(max) 10 nmol/min PubMed

    • Modification

  • autophosphorylation on Ser-60, Thr-55, Thr-62, and Thr-64 PubMed

    • Cofactors

  • Mg2+ PubMed

    • Structure

  • 5MVR PubMed

    • Localization

  • cytoplasm with the exception of the nucleoid region PubMed

    • Additional information

  • ATPase activity is required for in vivo function of the protein PubMed

    • Expression and Regulation

    Operons

    Genes
    thiL-tsaE-tsaB-ydiD-tsaD
    Description
    PubMed
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    Biological materials

    Mutant

  • BKE05910 (ΔtsaE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGCTTCACACGAGTCACCC, downstream forward: _UP4_ATGCTTTGTGAGGAGTTAAG
  • BKK05910 (ΔtsaE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGCTTCACACGAGTCACCC, downstream forward: _UP4_ATGCTTTGTGAGGAGTTAAG

    • Expression vectors

  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP833, available in Stülke lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP841, available in Stülke lab

    • References

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